Cryopreservation is the process of preserving cells, tissues, organs, or other biological constructs by cooling them to very low temperatures. This method is used widely in biology, medicine, and agriculture. The cryopreservation method is applied in various fields such as reproductive medicine, stem cell storage, tissue preservation, organ preservation, conservation biology, and research. The process of cryopreservation involves preparation, cooling, storage, and thawing.